Interest in a specialized microenvironment or "niche" regulating hemopoietic stem cell function has been steadily growing since the idea was first proposed by Ray Schofield over three decades ago. This growing interest, as well as more recently the interest in cellular-molecular-biochemical characterization of not only the hemopoietic stem cell niche but the niches for other stem cells, incited the compilation of Stem Cell Niche: Methods and Protocols. In this volume, scientists have provided protocols that will provide both a flavor of the field and hopefully stimulate new approaches and methodologies by those interested in the stem cell niche. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Stem Cell Niche: Methods and Protocols seeks to serve both experts and novices in the stem cell field with well-established protocols on this exciting subject.  

Inhaltsangabe1. Immunostaining of Germline Stem Cells and the Niche in Drosophila Ovaries            Lichao Luo, Phing Chian Chai, and Yu Cai 2. Identification of Male and Female Germline Stem Cells in DrosophilaShree Ram Singh, Madhuri Kango-Singh, and Eviatar Nevo 3. Visualization of Adult Stem Cells Within their Niches Using the Drosophila Germline as a Model System            Annekatrin König and Halyna R. Shcherbata 4. Morphometric Evaluation of the Spermatogonial Stem Cell Distribution and Niche in Vertebrates            Paulo Henrique Almeida Campos-Junior, Guilherme Mattos Jardim Costa, Gleide Fernandes de Avelar, Tânia Mara Segatelli, Samyra Maria Santos Nassif Lacerda, Pedro Manuel Aponte, and Luiz Renato de França 5. In vitro Construction of 2D and 3D Simulations of the Murine Hematopoietic Niche            Brahmananda Reddy Chitteti, Monique Bethel, Sherry L. Voytik-Harbin, Melissa A. Kacena, and Edward F. Srour 6. Isolation of Embryonic Hematopoietic Niche Cells by Flow Cytometry and Laser Capture Micro-Dissection            Daisuke Sugiyama and Tatsuya Sasaki 7. Isolation and Enrichment of Stro-1 Immunoselected Mesenchymal Stem Cells from Adult Human Bone Marrow            Emma L. Williams, Kate White, and Richard O.C. Oreffo            8. Primary Marrow Derived Stromal Cells: Isolation and Manipulation            Aravind Ramakrishnan, Beverly Torok-Storb, and Manoj M. Pillai 9. Detection In vitro and Quantitative Estimation of Artificial Microterritories Which Promote Osteogenic Differentiation and Maturation of Stromal Stem Cell             Igor A. Khlusov, Natalya M. Shevtsova, and Marina Yu 10. The Prospective Isolation of Viable, High Ploidy Megakaryocytes from Adult Murine Bone Marrow by Fluorescence Activated Cell Sorting            Yi Shen, Brenda Williams, Melonie J. Storan, and Susan K. Nilsson 11. Looking for the Niche: Substance Delivery into the Lateral Ventricle of the Brain: The Osmotic Minipump System            María Victoria Gómez Gaviro, Pedro Luis Sánchez Fernández, Robin Lovell Badge, and Francisco Fernández Avilés 12. Unbiased Stereological Method to Assess Proliferation Throughout the Subependymal ZoneAna Mendanha Falcão, Joana Almeida Palha, Ana Catarina Ferreira, Fernanda Marques, Nuno Sousa and João Carlos Sousa   13. Cardiac Stem Cell Niche, MMP9, and Culture and Differentiation of Embryonic Stem Cells Paras Kumar Mishra, Nicholas John Kuypers, Shree Ram Singh, Leiberh Noel Diaz, Vishalakshi Chavali, and Suresh C. Tyagi  14. Human and Murine Skeletal Muscle Reserve Cells            Rana Abou-Khalil, Fabien Le Grand, and Bénédicte Chazaud 15. Modulation of the Host Skeletal Muscle Niche for Donor Satellite Cell Grafting            Luisa Boldrin and Jennifer E. Morgan 16. Isolation of ckit+ Human Amniotic Fluid Stem Cells from Second Trimester            Michela Pozzobon, Martina Piccoli, Andrea Alex Schiavo, Anthony Atala, and Paolo De Coppi 17. Hypoxia and Visualization of the Stem Cell Niche            Pramudita R Ali Dalloul 18. Detection and Isolation of Human Disseminated Tumor Cells in the Murine Bone Marrow Stem Cell Niche             Yusuke Shiozawa, Russell S. Taichman, and Evan T. Keller 19. Identification and Separation of Normal Hematopoietic Stem Cells and Leukemia Stem Cells from Patients with Acute Myeloid Leukemia             Van T. Hoang, Isabel Taubert, Karina Borowski, Abraham Zepeda-Moreno, Dan Ran, Eike C. Buss, Patrick Wuchter, Volker Eckstein, and Anthony D. Ho 20. Serial Orthotopic Transplantation of Epithelial Tumors in Single-Cell Suspension            Heather A. McCauley